Cell culture microdevice

A cell culture microdevice for maintaining and culturing a cell therein comprising;

• a cell culture unit having at least a first cell carrier unit defining a cell culture chamber formed therein,
• the first cell carrier unit formed from at least,
• a chamber base shaped to support the cell thereon, and one or more chamber walls having one or more chamber wall surfaces enclosing the cell culture chamber about a chamber boundary,
• the first cell carrier unit further providing a guiding surface to guide instruments or fluids into the cell culture chamber located at an aperture through a chamber wall,
• wherein the dimensions of the cell culture microdevice are between approximately 0.1 μm and approximately 1000 μm in width, height and length,
• wherein the cell culture microdevice is configured to be maintained in a liquid medium within a larger vessel and to enable the passive exchange of liquid fluids with the larger vessel, and
• the cell culture microdevice is configured at a scale to substantially enclose a single cell or cell mass therein.