Modular DNA-binding domains and methods of use
AssigneesUlla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
InventorsUlla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
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Abstract
The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.
Claims15
A nucleic acid molecule encoding a protein comprising an artificial transcription activator-like (TAL) effector repeat region,
- wherein the artificial TAL effector repeat region comprises a plurality of contiguous repeat units and
- wherein the protein is capable of recognizing a predetermined nucleotide sequence in an animal cell.
A non-human animal cell or an in vitro human cell comprising a nucleic acid molecule or the protein encoded by the nucleic acid molecule, wherein the nucleic acid molecule encodes a protein comprising an artificial transcription activator-like (TAL) effector repeat region,
- wherein the artificial TAL effector repeat region comprises a plurality of contiguous repeat units and
- wherein the protein is capable of recognizing a predetermined nucleotide sequence in an animal cell.
A nucleic acid molecule encoding a protein comprising a modified TAL effector repeat domain comprising repeat units having one amino acid or two adjacent amino acids per repeat unit which determine(s) selective recognition of a base pair in a DNA sequence, wherein the protein is capable of specifically recognizing an endogenous target DNA sequence in an animal cell.
A non-human animal cell or an in vitro human cell comprising a nucleic acid molecule or the protein encoded by the nucleic acid molecule, wherein the nucleic acid molecule encodes a protein comprising a modified TAL effector repeat domain comprising repeat units having one amino acid or two adjacent amino acids per repeat unit which determine(s) selective recognition of a base pair in a DNA sequence, and wherein the protein is capable of specifically recognizing an endogenous target DNA sequence in an animal cell.
Dependencies are inferred from the claim text.