Use of anti-aging glycoprotein for enhancing survival of neurosensory precursor cells

A method for protecting isolated neurosensory precursor cells from prostaglandin E2 toxicity comprising the step of:

• a) contacting an isolated neurosensory precursor cell with a gem-difluorinated C-glycopeptide compound of general formula I, or a pharmaceutically acceptable base, addition salt with an acid, hydrate or solvate thereof, and then washing the isolated neurosensory cells to remove the compound:
• in which:
• N is an integer between 1 and 5,
• R4═H, AA1, or AA1-AA2,
• R5═OH, AA1, or AA1-AA2,
• AA1 and AA2 independently represent amino acids with a non-polar side chain
• and
• R1, R2, R3 are independent groups in which two of R1, R2 and R3 are selected from H, CH3, CH2Ph, CH(CH3)2, CH2CH(CH3)2 or CH(CH3)CH2CH3 and the remaining R1, R2, R3 is
• in which:
• n is an integer between 3 and 4,
• Y, Y′ are independent groups
• in which Y, Y′═H, OR, N3, NR′R″, or SR′″,
• where R═H, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl or acetate group,
• R′, R″ independently=H, alkyl, allyl, benzyl, tosylate group, C(═O)-alkyl or C(═O)-Bn,
• R′″═H, alkyl, or acetate group,
• R6 is H, CH3, CH2OH, CH2-glycoside group or CH2—OGP in which GP is a protector group selected from alkyl, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R7═OH, OGP′, NH2, N3, NHGP′ or NGP′GP″ in which GP′ and GP″ are independently selected from alkyl, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R8 is a hydrogen atom H or a free or protected alcohol function,
• and
• if R1═R2═H, CH3, CH2Ph, CH(CH3)2, CH2CH(CH3)2, or CH(CH3)CH2CH3
• then
• in which:
• n is an integer between 3 and 4,
• Y, Y′ are independent groups
• in which Y, Y′═H, OR, N3, NR′R″, or SR′″,
• where R═H, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R′, R″ independently=H, alkyl, allyl, benzyl, tosylate group, C(═O)-alkyl, or C(═O)-Bn,
• R′″═H, alkyl, or acetate group,
• R6 is H, CH3, CH2OH, CH2-glycoside group, or CH2—OGP in which GP is a protector group selected from alkyl, benzyl, trimethylsilyl, tertbutyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R7═OH, OGP′, NH2, N3, NHGP′, or NGP′GP″ in which GP′ and GP″ are independently selected from alkyl, benzyl, trimethylsilyl, tertbutyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R8 is a hydrogen atom H or a free or protected alcohol function,
• if R1═R3═H, CH3, CH2Ph, CH(CH3)2, CH2CH(CH3)2, or CH(CH3)CH2CH3
• then
• in which:
• n is an integer between 3 and 4,
• Y, Y′ are independent groups
• in which Y, Y′═H, OR, N3, NR′R″, or SR′″,
• where R═H, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R′, R″ independently=H, alkyl, allyl, benzyl, tosylate group, C(═O)-alkyl, or C(═O)-Bn,
• R′″═H, alkyl, or acetate group,
• R6 is H, CH3, CH2OH, CH2-glycoside group, or CH2—OGP in which GP is a protector group selected from alkyl, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R7═OH, OGP′, NH2, N3, NHGP′, or NGP′GP″ in which GP′ and GP″ are independently selected from alkyl, benzyl, trimethylsilyl, tertbutyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R8 is a hydrogen atom H or a free or protected alcohol function,
• if R2═R3═H, CH3, CH(CH3)2, CH2CH(CH3)2, or CH(CH3)CH2CH3
• then
• in which:
• n is an integer between 3 and 4,
• Y, Y′ are independent groups
• in which Y, Y′H, OR, N3, NR′R″, or SR′″,
• where R═H, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R′, R″ independently=H, alkyl, allyl, benzyl, tosylate group, C(═O)-alkyl, or C(═O)-Bn,
• R′″═H, alkyl, or acetate group,
• R6 is H, CH3, CH2OH, CH2-glycoside group, or CH2—OGP in which GP is a protector group selected from alkyl, benzyl, trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R7═OH, OGP′, NH2, N3, NHGP′, or NGP′GP″ in which GP′ and GP″ are independently selected from alkyl, benzyl, trimethylsilyl, tertbutyldimethylsilyl, tert-butyldiphenylsilyl, or acetate group,
• R8 is a hydrogen atom H or a free or protected alcohol function
• b) transplanting the treated isolated neurosensory precursor cells of step a) in a subject in need thereof,
• wherein said compound protects said isolated neurosensory precursor cell from prostaglandin E2 toxicity and wherein the method comprises no step where said isolated neurosensory precursor cells are cryopreserved.