Polypeptide and nucleic acid capable of changing amylose content (AC) in plant, and use thereof
AssigneeShandong Shunfeng Biotechnology Co., Ltd.
InventorsJinshan Zhang, Xiaorui Huang, Xiaomu Niu
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Abstract
A mutant granule-bound starch synthase 1 (GBSS1) polypeptide and a nucleic acid, and use thereof are provided. Compared to an amino acid sequence of a parent GBSS1, the mutant GBSS1 polypeptide has a mutation at an amino acid corresponding to one or more of amino acid 237, amino acid 168, and amino acid 411 of an amino acid sequence shown in SEQ ID NO: 1. An amylose content (AC) in a plant changes after the plant undergoes GBSS1 mutation, which has very promising application prospects in the improvement of edible quality of rice.
Claims19
A mutant granule-bound starch synthase 1 (GBSS1) polypeptide, wherein compared to an amino acid sequence of a parent GBSS1 as shown in SEQ ID NO:
- 1, the mutant GBSS1 comprises:
- a mutation at an amino acid corresponding to one or more of amino acid 237 and/or amino acid 168 wherein the amino acid residue 237 is mutated into alanine (A) and/or the amino acid residue 168 is mutated into leucine (L).
A polynucleotide encoding a mutant GBSS1 polypeptide, wherein compared to a nucleic acid sequence of a parent GBSS1 as shown in SEQ ID NO:
- 8, the mutant GBSS1 comprises:
- a mutation encoding an amino acid corresponding to one or more of amino acid 237 and/or amino acid 168, wherein the amino acid residue 237 is mutated into alanine (A) and/or the amino acid residue 168 is mutated into leucine (L).
Dependencies are inferred from the claim text.