Chromatographic separation method and chromatographic separation device

A chromatographic separation method for separating a plurality of components contained in a liquid, with a separating device including a plurality of filling portions filled with a separating agent for separating the plurality of components contained in the liquid,

• a supply portion provided in each of the plurality of filling portions to supply the liquid or an eluent for extracting any component contained in the liquid to the filling portion, and
• an extraction portion provided in each of the plurality of filling portions to extract any component contained in the liquid from the filling portion,
• the method comprising:
• an upstream supplying and extracting step of extracting any component contained in the liquid from an upstream stream extraction portion while supplying the eluent to at least one filling portion from an upstream stream supply portion by an upstream stream;
• a downward supplying and extracting step of supplying each of the liquid and the eluent to different filling portions of the plurality of filling portions from the supply portion by a downward stream and of extracting a separated liquid from any of the extraction portions;
• and
• a circulating step of circulating the liquid and the eluent in the filling portion between the filling portions by a downward stream, without supplying the liquid and the eluent.