Method, reagent, and chip for measuring analyte concentration in specimen
AssigneeTerumo Kabushiki Kaisha
InventorsFumihiko Chai, Takeyuki Moriuchi
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Abstract
Provided is a means capable of measuring an analyte concentration with high accuracy without requiring fractionation of a biological component. The present invention relates to a method for measuring an analyte concentration in a specimen, including (1) preparing the specimen, (2) mixing a coloring reagent, an oxidoreductase, and a light-scattering adjustment reagent with the specimen to obtain an analysis sample having substantially the same scattered light as the specimen, and (3) measuring the analyte concentration by using the analysis sample.
Claims15
A method for measuring an analyte concentration in a specimen, comprising:
- (1) providing the specimen;
- (2) mixing the specimen with a coloring reagent, an oxidoreductase, and a light-scattering adjustment reagent, and a transition metal compound to obtain an analysis sample having substantially the same scattered light as the specimen, wherein a mixing ratio of the light-scattering adjustment reagent to a transition metal compound (defined as mol of the light-scattering adjustment reagent to 1 mol of the transition metal compound) is more than 0 mol and is 0.24 mol or less;
- and
- (3) measuring the analyte concentration by using the analysis sample.
An analyte concentration measuring reagent comprising:
- a coloring reagent;
- an oxidoreductase;
- a light-scattering adjustment reagent;
- and
- a transition metal compound at a ratio of 4.0 mol or more with respect to 1 mol of the coloring reagent.
An analyte concentration measuring reagent, comprising:
- a coloring reagent comprising 2-substituted benzothiazolyl-3-substituted phenyl-5-substituted sulfonated phenyl-2H-tetrazolium salt represented by the following formula (2):
- wherein R1 is any one selected from the group consisting of a hydrogen atom, a hydroxy group, a methoxy group, and an ethoxy group;
- R2 is any one selected from the group consisting of a nitro group, —OR4, and a carboxyl group;
- R3 is a hydrogen atom, a methyl group or an ethyl group, at least one of R3 is a methyl group or an ethyl group;
- R4 is a methyl group or an ethyl group;
- m is a number in which the sulfo group (—SO3−) is bonded to the phenyl group at the 5-position of the tetrazole skeleton, and is 1 or 2;
- n is a number in which R2 is bonded to the phenyl group at the 3-position of the tetrazole skeleton, and is an integer of 0 to 2;
- p is a number in which the sulfo group (—SO3−) is bonded to the phenyl group at the 3-position of the tetrazole skeleton, and is 0 or 1;
- n+p is 1 or more;
- q is 1 or 2, and when q is 2, each OR3 is arranged adjacent to each other, wherein each OR3 may form a ring with each other;
- and X represents a hydrogen atom or an alkali metal;
- an oxidoreductase;
- a light-scattering adjustment reagent comprising an aromatic hydrocarbon having at least one sulfonic acid group or a salt thereof;
- and
- a transition metal compound at a ratio of 4.0 mol or more with respect to 1 mol of the coloring reagent.
Dependencies are inferred from the claim text.