Engineered materials and methods of forming

A method of forming a textile, the method comprising:

• mapping a three dimensional spatial distribution of at least one structural protein fiber of extracellular matrix of biological material of interest comprising collagen fibers and elastin fibers, the three dimensional spatial distribution using second harmonic imaging microscopy imaging of the collagen fibers and two photon excitation imaging microscopy of the elastin fibers to generate individual tiles in a transverse x-y plane of an x-y-z coordinate system;
• stacking the individual tiles in the z-direction to form a three-dimensional model;
• designing a fiber assembly pattern comprising a weaving algorithm based on an intrinsic pattern of the at least one structural protein fiber of the extracellular matrix of the biological material in the three-dimensional model;
• and
• assembling fibers based on the fiber assembly pattern to form the textile.

A method of forming an engineered tissue construct, the method comprising:

• mapping a three dimensional spatial distribution of collagen fibers and elastin fibers of the extracellular matrix of a tissue of interest, the three dimensional spatial distribution using second harmonic imaging microscopy imaging of the collagen fibers and two photon excitation imaging microscopy of the elastin fibers to generate individual tiles in a transverse x-y plane of an x-y-z coordinate system;
• stacking the individual tiles in the z-direction to form a three-dimensional model;
• designing a fiber weaving motif based on an intrinsic pattern of the collagen fibers and elastin fibers of the extracellular matrix of the tissue in the three-dimensional model;
• and
• weaving fibers based on the fiber weaving motif to define the weave pattern and fiber orientation and form the engineered tissue construct.