US 11,814,663 B2 · Microorganisms with improved 1,3-propanediol and butyric acid production

Abstract

The present invention concerns a new mutant strain of Clostridium acetobutylicum comprising attenuated glycerol kinase activity. In addition, the present invention concerns a consortium of Clostridium comprising at least said mutant strain and at least one other species of Clostridium chosen among C. sporogenes and C. sphenoides. As this modified strain may be adapted for growth and for the production of 1,3-propanediol in an appropriate culture medium with high glycerol content, the invention also relates to a method for the production of 1,3-propanediolandbutyric acid, by culturing at least this mutant strain in an appropriate culture medium.

Claims19

Dependent claims

A mutant strain of Clostridium acetobutylicum expressing dhaB1, dhaB2 and dhaT genes from C. butyricum and comprising attenuated glycerol kinase activity, wherein the glycerol kinase is encoded by gene glpK with SEQ ID NO:

2 comprising at least one of the following mutations:
a. a mutation leading to a mutated protein with SEQ ID NO:
3,
b. a mutation leading to a mutated protein with SEQ ID NO:
5,
c. a mutation leading to a mutated protein with SEQ ID NO:
7.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/267,347Filed 9 Aug 2019

Priority

EP 1830609910 Aug 2018

Published

US 2021/0254108 A119 Aug 2021

Granted

14 Nov 2023

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12P 7/52
Containing a carboxyl group including Peroxycarboxylic acids: propionic acid; Butyric acids
C07K 14/33
From bacteria: from Clostridium
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 9/0067
Oxidoreductases: acting on hydrogen as donor

Microorganisms with improved 1,3-propanediol and butyric acid production

Life of this patent

Microorganisms with improved 1,3-propanediol and butyric acid production

Life of this patent

Abstract

Claims19