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US 11,814,659 B2Granted
Tunable nanoscale cages from self-assembling DNA and protein building blocks
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsNicholas Stephanopoulos, Yang Xu
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Abstract
Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.
Claims6
A three-dimensional nanocage comprising:
- a protein covalently linked to a polynucleotide handle, wherein the protein is a homotrimer;
- wherein the polynucleotide handle is covalently linked to a solvent exposed cysteine by a disulfide linkage or
- wherein the polynucleotide handle is covalently linked to a surface 4-azidophenylalanine residue;
- and
- wherein the polynucleotide handle is at least 15 base pairs long;
- and
- a DNA assembly comprising a polynucleotide arm complementary to the polynucleotide handle linked to the protein, wherein the polynucleotide handle and the polynucleotide arm form a double-stranded complex linked to the protein to the DNA assembly;
- and
- wherein the DNA assembly comprises 4 oligonucleotides.
6 more elements
Dependencies are inferred from the claim text.