Retroviral and lentiviral vectors
AssigneeAutolus Limited
InventorsMartin Pulé, Leila Mekkaoui
Life of this patent
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Abstract
The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises: (i) a mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain and a transmembrane domain; and/or (ii) a cytokine-based T-cell activating transmembrane protein which comprises a cytokine domain and a transmembrane domain, wherein the mitogenic or cytokine-based T-cell activating transmembrane protein is not part of a viral envelope glycoprotein. When cells such as T-cells of Natural Killer cells are transduced by such a viral vector, they are simultaneously activated by the mitogenic T-cell activating transmembrane protein and/or the cytokine-based T-cell activating transmembrane protein.
Claims20
A retroviral or lentiviral vector having a viral envelope which comprises a cytokine-based T-cell activating transmembrane protein which comprises a cytokine domain and a transmembrane domain,
- wherein the cytokine-based T-cell activating transmembrane protein is not part of a viral envelope glycoprotein.
A packaging cell which expresses, at the cell surface,
- a cytokine-based T-cell activating transmembrane protein which comprises a cytokine domain and a transmembrane domain and which also comprises one or more of the following genes:
- gag, pol, env and/or rev.
A producer cell which expresses, at the cell surface,
- a cytokine-based T-cell activating transmembrane protein which comprises a cytokine domain and a transmembrane domain and which comprises gag, pol, env and optionally rev genes and also comprises a retroviral or lentiviral genome.
A kit for making a packaging cell which comprises:
- (i) one or more nucleic acid(s) encoding a cytokine-based T-cell activating transmembrane protein and
- (ii) nucleic acids comprising retroviral gag, pol, env and optionally rev genes.
A kit for making a producer cell which comprises:
- (i) one or more nucleic acid(s) encoding a cytokine-based T-cell activating transmembrane protein,
- (ii) nucleic acids comprising retroviral gag, pol, and env and optionally rev genes, and
- (iii) a retroviral or lentiviral vector genome.
Dependencies are inferred from the claim text.