Genetically engineered foot and mouth disease virus and related proteins, polynucleotides, compositions, methods and systems
AssigneesLawrence Livermore National Security, LLC, The United States of America as represented by the Secretary of Agriculture, University of Connecticut
InventorsAida E. Rieder, Teresa B. De Los Santos, Luis L. Rodriguez, Devendra Rai, Fayna C. Diaz-San Segundo, Paul D. Hoeprich
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Abstract
Genetically engineered Foot and Mouth Disease Virus (FMDV) and related engineered proteins and polynucleotides, nanolipoprotein particles, compositions, methods and systems are described. The genetically engineered FMDV is modified by the strategic insertion of a protein tag into select regions of the FMDV genome which encode viral proteins that are exposed on the surface of the FMDV viral capsid. The inserted protein tag is displayed as a decoration or attachment on the viral capsid surface.
Claims27
A vaccine comprising
- (i) one or more genetically modified Foot and Mouth Disease Virus (FMDV) having a viral capsid comprising
- a protein tag up to 100 amino acids inserted in an engineered FMDV VP1 protein and presented on an external surface of the viral capsid,
- and/or
- (ii) one or more FMD functionalized nanolipoprotein particle comprising a scaffold protein and a functionalized membrane forming lipid presenting
- a tag substrate attached to the protein tag presented on the external surface of the viral capsid of the one or more genetically modified FMDV,
- together with
- (iii) a pharmaceutically acceptable vehicle,
- wherein the protein tag is inserted in the engineered FDMV VP1 protein at an insertion position selected from
- any one of position −1 to position +6 relative to an FMDV 2A N-terminus of the engineered FDMV VP1 protein,
- and
- any one of position −1 to position −7 relative to an FMD VP1 C-terminus of the engineered FDMV VP1 protein.
Dependencies are inferred from the claim text.