Brassica events LBFLFK and LBFDAU and methods for detection thereof
AssigneeBASF Plant Science GmbH
InventorsToralf Senger, Laurent Marty, Irene Kunze, Dietrich Rein, Jörg Bauer, Carl Andre
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Abstract
The present invention provides transgenic Brassica events LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events. The invention also provides artificial oligonucleotide primers and probes that are diagnostic for the LBFLFK and LBFDAU events and their progeny in a sample, and methods for detecting the presence of the LBFLFK and LBFDAU events and their progeny in a sample. The invention further provides oil and commodity products derived from the LBFLFK and LBFDAU events.
Claims6
A method of detecting the presence of DNA corresponding to Brassica event LBFLFK in a sample comprising DNA, the method comprising the steps of:
- (a) contacting the sample with an LBFLFK Locus 1 primer pair that, when used in a nucleic acid amplification reaction with genomic DNA from Brassica event LBFLFK, produces a Locus 1 amplicon that is diagnostic for Brassica event LBFLFK;
- (b) performing a nucleic acid amplification reaction, thereby producing the Locus 1 amplicon;
- and
- (c) detecting the amplicon, wherein the amplicon comprises the LBFLFK Locus 1 junction region SEQ ID NO:4 or SEQ ID NO:5, or the complement thereof.
A method of detecting the presence of DNA corresponding to Brassica event LBFLFK in a sample comprising DNA, the method comprising the steps of:
- (a) contacting the sample with an LBFLFK Locus 2 primer pair that, when used in a nucleic acid amplification reaction with genomic DNA from Brassica event LBFLFK, produces a Locus 2 amplicon that is diagnostic for Brassica event LBFLFK;
- (b) performing a nucleic acid amplification reaction, thereby producing the Locus 2 amplicon;
- and
- (c) detecting the amplicon, wherein the amplicon comprises the LBFLFK Locus 2 junction region SEQ ID NO:13 or SEQ ID NO:14, or the complement thereof.
Dependencies are inferred from the claim text.