Method of analyzing diluted biological sample component
AssigneeLeisure, Inc.
InventorsSusumu Osawa, Shinya Sugimoto, Isao Yonekubo
Life of this patent
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Abstract
There is provided a method of analyzing a biological sample component that allows easy and accurate quantification and counting of any of a plasma component and a blood cell component in a trace and unknown amount of a whole blood sample collected from a finger, for example. The method of the present invention is a method of analyzing a biological sample component in a trace amount of blood, comprising analyzing a diluent buffer into which the blood has been mixed and an internal standard substance and/or an external standard substance contained in the diluent buffer, calculating a dilution ratio, and analyzing a biological component in a plasma or serum component in the blood.
Claims7
A method of quantitative analysis of a component to be analyzed in a blood sample, the method comprising
- (a) diluting the blood sample with a diluent buffer to quantitatively analyze the component to be analyzed in blood, wherein
- a predetermined amount of an internal standard substance is added into the diluent buffer so that the internal standard substance has a predetermined concentration in the diluent buffer, and
- the internal standard substance is an element belonging to alkali metals or alkaline earth metals, and
- (b) measuring a concentration of the internal standard substance and a concentration of an external standard substance in the blood sample diluted with the diluent buffer, wherein
- the external standard substance is a component homeostatically comprised at a predetermined concentration in the blood sample and is selected from the group consisting of sodium, chloride, albumin, and total protein,
- the diluent buffer is free of the internal standard substance and the external standard substance in a state that the internal standard substance is not added into the diluent buffer,
- (c) determining a dilution factor of the blood sample based on measured values of the concentration of the internal standard substance and the concentration of the external standard substance, and
- (d) quantifying a biological component in the blood sample based on the determined dilution factor.
Dependencies are inferred from the claim text.