Systems and methods for an electrochemical total cholesterol test

A dry test strip and meter combination system for electrochemical testing of a blood analyte, the system comprising:

• a dry test strip including:
• a first receiving port, the first receiving port for receiving a blood sample, the first receiving port at a first end of the test strip, wherein a blood separation membrane is placed over the first receiving port, the blood separation membrane including lectins;
• a first electrode and a second electrode, the first and second electrodes proximate to the first receiving port, including a capillary tube at the first receiving port, wherein the capillary tube leads to the first electrode and the second electrode and in the capillary tube, a dried surfactant solution is located, whereby the dried surfactant solution is wetted by the blood sample;
• a first contact and a second contact, the first and second contacts at a second end of the test strip, the first and second contacts interconnected with the first and second electrodes, respectively;
• a meter, the meter having a test strip receiving port shaped to receive the test strip, the meter including a plurality of contacts, a first portion of the plurality of contacts positioned to interface with the first and second contacts;
• and
• a mediator and cholesterol oxidase configured to interact with the blood sample and the first and second electrode to produce a cholesterol level measured on the basis of a measurable electrical event detected by the meter and converted to the cholesterol level, wherein the mediator is an osmium compound;
• the dried surfactant solution including Sodium Cholate, Potassium Phosphate, and BSA (bovine serum alluvium), located proximate to the first and second electrode.