Systems and methods for pre-analytical substrate processing
AssigneeGenomic Health, Inc.
InventorsHubert Yeung, Amy Lee Hsieh Yuan, Chun Wai Lee, Gabriel Jesus Samuel Perlas Moraleda, Jonathan M. Cassel
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Abstract
Some embodiments presented in this disclosure concern an Automated Tissue Dissection (ATD) System. An ATD system is a one stop, and potentially low-cost, system to perform dissections on a substrate from pathologist digital mark or pen mark on the substrate using non-contact and/or mechanical method to extract a Formalin-Fixed Paraffin-Embedded (FFPE) tissue sample with: (a) only the ROI or ROIs as area to be saved; and (b) remove or decompose nucleic acid content in the region of no interest (RONI) and collect all tissue sample from a standard microscope substrate into a specific container.
Claims17
A method of processing a biological sample for a biological assay, comprising (a) contacting the biological sample with a contact medium comprising a particulate substance blasted with pressurized air under conditions sufficient to effectuate blasting of a region of non-interest (RONI) in the biological sample with blasted particulate substance, thereby causing at least partial transfer of the RONI to the contact medium;
- (b) removing the contact medium from the biological sample;
- and (c) dissecting a region of interest (ROI) in the biological sample or collecting the remaining biological sample.
A method of assaying for an analyte in a biological sample comprising processing the biological sample by (a) contacting the biological sample with a contact medium comprising a particulate substance blasted with pressurized air under conditions sufficient to effectuate blasting of a region of non-interest (RONI) in the biological sample with blasted particulate substance thereby causing at least partial transfer of the RONI to the contact medium;
- (b) removing the contact medium from the biological sample to obtain a processed biological sample;
- (c) dissecting the processed biological sample or collecting the entire process biological sample;
- and (d) assaying for the analyte in the processed biological sample.
Dependencies are inferred from the claim text.