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US 11,807,873 B2Granted
Conversion of S-lignin compounds to useful intermediates
AssigneeAlliance for Sustainable Energy, LLC
InventorsChristopher W. Johnson, Allison Jean Zimont Werner, Gregg Tyler Beckham, Sandra Fabienne Notonier
Life of this patent
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Abstract
The present disclosure relates to a genetically modified microbial cell that includes a first genetic modification resulting in the expression of an exogenous vanillate demethylase, such that the microbial cell is capable of metabolizing an S-lignin decomposition product and producing 2-pyrone-4,6-dicarboxylate (PDC).
Claims16
A genetically modified Pseudomonas sp. comprising:
- a first genetic modification of a first gene encoding an exogenous vanillate demethylase, wherein the first gene has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 11 or SEQ ID NO:
- 13, and wherein the expressed exogenous vanillate demethylase has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 12 or SEQ ID NO:
- 14;
- and
- a second genetic modification of a second gene encoding an exogenous protocatechuate dioxygenase, wherein the second gene has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 5 or SEQ ID NO:
- 7, and wherein the exogenous protocatechuate dioxygenase has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 6 or SEQ ID NO:
- 8;
- wherein:
- the Pseudomonas sp. metabolizes a S-lignin decomposition product, and
- the Pseudomonas sp. produces 2-pyrone-4,6-dicarboxylate (PDC).
11 more elements
A method for lignin valorization, the method comprising:
- converting an S-lignin decomposition product to 2-pyrone-4,6-dicarboxylate (PDC) utilizing a genetically modified Pseudomonas sp., wherein:
- the genetically modified Pseudomonas sp. comprises:
- a first genetic modification of a first gene encoding an exogenous vanillate demethylase, wherein the first gene has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 11 or SEQ ID NO:
- 13 and wherein the expressed exogenous vanillate demethylase has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 12 or SEQ ID NO:
- 14;
- and
- a second genetic modification of a second gene encoding an exogenous protocatechuate dioxygenase, wherein the second gene has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 5 or SEQ ID NO:
- 7 and wherein the exogenous protocatechuate dioxygenase has a sequence that is at least 80% identical to at least one of SEQ ID NO:
- 6 or SEQ ID NO:
- 8.
10 more elements
Dependencies are inferred from the claim text.