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US 11,806,445 B2Granted
Multi-layer skin substitute products and methods of making and using the same
AssigneeWake Forest University Health Sciences
InventorsAnthony Atala, Gayoung Jeong, James J. Yoo, Sang Jin Lee, Young-Joon Seol
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Abstract
Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics.
Claims18
A method of making a skin substitute product, comprising the steps of:
- (a) depositing a first layer comprising live mammalian adipocytes in a first hydrogel carrier on a substrate;
- then
- (b) depositing a second layer on said first layer, said second layer comprising live mammalian fibroblast cells and live mammalian follicle dermal papilla cells in a second hydrogel carrier;
- and then
- (c) depositing a third layer on said second layer, said third layer comprising live mammalian keratinocytes and live mammalian melanocytes in a third hydrogel carrier,
- wherein said live mammalian adipocytes are human adipocytes, said live mammalian fibroblast cells are human fibroblast cells, said live mammalian follicle dermal papilla cells are human follicle dermal papilla cells, said live mammalian keratinocytes are human keratinocytes, and said live mammalian melanocytes are human melanocytes.
3 more elements
A method of making a skin substitute product, comprising the steps of:
- (a) depositing a first layer comprising live mammalian adipocytes in a first hydrogel carrier on a substrate;
- then
- (b) depositing a second layer on said first layer, said second layer comprising live mammalian fibroblast cells and live mammalian follicle dermal papilla cells in a second hydrogel carrier;
- and then
- (c) depositing a third layer on said second layer, said third layer comprising live mammalian keratinocytes and live mammalian melanocytes in a third hydrogel carrier,
- said method further comprising depositing antigen-presenting dendritic cells or precursors thereof between said first layer and said second layer, in said second layer, between said second layer and said third layer, and/or in said third layer.
3 more elements
Dependencies are inferred from the claim text.