Methods for genetic modification of plants
AssigneeCeres, Inc.
InventorsRoger I. Pennell, Richard Hamilton, Delin Liang
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Abstract
Described are methods and materials for the genetic modification of plants by specific gene targeting and precise editing of nucleic acid sequences in a plant. The methods and materials provided herein enable one to edit the plant genome by design to control the expression of endogenous genes and/or control the transmission and expression of transgenic traits. Provided are also methods of producing plants having a desirable agronomic trait by crossing a transgenic plant expressing a gRNA with a plant expressing a Cas enzyme, and selecting a progeny plant having the desirable agronomic trait or a seed thereof.
Claims9
A transgenic plant or transgenic plant seed comprising a nucleic acid molecule comprising a reproductive tissue specific promoter operably linked to a heterologous transgene that encodes a Cas enzyme, wherein the reproductive tissue specific promoter is floral meristem promoter selected from the group consisting of:
- a promoter of a Zap1a, Zap1b, ZLF1, ZLF2, or ZMM4 endogenous gene,
- a nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO:
- 16, and
- a nucleic acid molecule comprising a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 16 or a fragment of SEQ ID NO:16 having the promoter activity of SEQ ID NO:
- 16.
Dependencies are inferred from the claim text.