Increasing plant oil content by improving activity of acetyl-CoA carboxylase
AssigneeThe Curators of the University of Missouri
InventorsJay J. Thelen, Matthew Salie
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Abstract
The present invention provides a method and means to change fatty acid and ultimately triacylglycerol production in plants and algae. Methods of the invention comprise the step of altering the activity levels of the committed step for de novo fatty acid biosynthesis, acetyl-CoA carboxylases (ACCase). More specifically, methods of the invention directly enhance the activity of ACCase by overexpression of α-CT or a catalytic portion thereof.
Claims8
A method of altering fatty acid and/or triacylglycerol production in plants and/or algae, comprising the step of altering activity levels of the committed step for de novo fatty acid biosynthesis, acetyl-CoA carboxylase (ACCase), wherein said altering step comprises increasing the activity level of ACCase by increasing expression of α-carboxyltransferase (α-CT) or a catalytic portion thereof, and total or partial silencing of one or more biotin/lipoyl attachment domain containing (BADC) gene, wherein said BADC gene comprises from about 90% to 100% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, or 6, or a complement thereof, in said plant and/or algae, and wherein said increased activity level of ACCase and said increased expression of α-CT is compared to a control plant and/or algae prior to altering activity levels of ACCase.
Dependencies are inferred from the claim text.