Methods for assessing cell surface glycosylation
AssigneeJuno Therapeutics, Inc.
InventorsPaul Ken Kodama, Tom Kowski, Mima Mujacic, Kenneth Mayo Prentice
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Abstract
Provided herein are methods for assessing cell surface glycans, e.g., N-glycans, by assessing a sample of released surface glycans, and determining the presence, absence, or level of glycans present in the sample. Also provided are methods of assaying and/or evaluating a cell composition by assessing the cell surface glycan profile of the cell composition and comparing the profile to a reference sample. Methods for manufacturing and/or culturing a plurality of cell compositions having consistent surface glycan expression with low variability are also provided.
Claims28
A method for assessing cell surface glycans, the method comprising:
- (a)(i) incubating a test composition comprising a plurality of cells under conditions to release one or more glycans from the surface of cells in the test composition, wherein a sample comprising one or more cell surface glycans is generated and (a)(ii) determining the presence, absence, identity and/or level of glycans present in the sample, thereby assessing the cell surface glycan profile of the sample;
- or
- (b) determining the presence, absence, identity and/or level of glycans present in a sample, thereby assessing the cell surface glycan profile of the sample, wherein the sample comprises one or more glycans released from the surface of cells present in a test composition comprising a plurality of cells after incubation of the test composition under conditions to release the one or more glycans;
- and
- wherein the cells express a recombinant receptor or the test composition comprises cells expressing a recombinant receptor.
Dependencies are inferred from the claim text.