Back to results
US 11,795,491 B2Granted
Photoactivatable antimicrobial agents and therapeutic and diagnostic methods of using same
AssigneeThe General Hospital Corporation
InventorsTayyaba Hasan, Ulysses W. Sallum, Sarika Verma, Gerard Nau
Life of this patent
Estimating the term…
Abstract
The present invention provides photosensitizer compounds for use in detecting beta-lactamase activity. Methods and kits that utilize the photosensitizer compounds of the invention for the detection of, quantitation of, and classification or typing of microbial beta-lactamases.
Claims6
A method of measuring susceptibility of bacteria in a sample to a beta-lactam antibiotic, wherein the method comprises:
- (a) contacting the sample with:
- (i) a composition comprising a compound according to formula I:
- X-L-X′,
- wherein L is a cephalosporin linker or fragment thereof, and both X and X′ are bodipy dyes, wherein the bodipy dyes are quenched by a static quenching mechanism when L is uncleaved, to form a standard;
- and
- (ii) a composition comprising the beta-lactam antibiotic and the composition of (i), to form a test sample;
- (b) light-activating the standard and the test sample to produce a fluorescence signal;
- (c) quantifying the fluorescence signal with a detector to obtain a result;
- (d) comparing the test sample result to the standard result;
- and
- (e) if the amount of the measured fluorescence in the test sample is less than the amount of measured fluorescence in the standard, then the bacteria is determined to be susceptible to the β-lactam antibiotic.
8 more elements
Dependencies are inferred from the claim text.