# US 11,795,489 B2 · Low cross-linking gelatine

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- Number: US 11,795,489 B2
- Document ID: US11795489B2
- Status: Granted
- Kind: B2
- Application number: 16/319,679
- Filed: 2017-07-24
- Published: 2020-12-03
- Granted: 2023-10-24
- Earliest claimed: 2016-07-22
- Assignees: Rousselot B.V.
- Applicants: Rousselot B.V.
- Inventors: Pierre-Albert Marie Thomas; Claude Françoise Capdepon; Ellen Leona Gabriël Verwee; Catherine Emmanuelle Thérèse Baron
- Web page: https://patentgrep.com/patent/US11795489B2

## CPC

- C12P 21/06: produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00)
- A61K 9/4825: {Proteins, e.g. gelatin (gelatin capsule shells with substantial amounts of other macromolecular substances A61K9/4816)}
- A61K 9/70: Web, sheet or filament bases {; Films; Fibres of the matrix type containing drug (hollow drug-filled fibres A61K9/0092)}
- C12N 2533/54: Collagen; Gelatin
- C12Y 304/21067: Endopeptidase So (3.4.21.67)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

The present invention relates to a process for the preparation of a gelatine blend comprising the steps of a) providing a gelatine extract or a series of gelatine extracts, differing in bloom and viscosity; b) subjecting a portion of one or more of the gelatine extracts of a) to an enzymatic treatment to obtain one or more enzymatically treated extracts having a viscosity that is lower than that of the respective extract before said enzymatic treatment, while maintaining the bloom substantially unaffected, the enzymatically treated extracts comprising less than 1.5 w/w % of gelatine-derived peptides having a molecular weight of less than 2000 Da; c) selecting one or more gelatine extracts of step a); d) combine the selected one or more gelatine extracts of step c) and blend with one or more enzymatically treated extracts of step b) to provide a gelatine composition having a lower viscosity as compared to that of the said combined selected extracts of step c) without being blended with said selected one or more enzymatically treated gelatine extracts of step b), while maintaining the bloom substantially unaffected. The present invention also relates to a gelatine composition, and a gelatine product comprising said gelatine composition.

## Claims

### Claim 1

A process for the preparation of a gelatine blend comprising the steps of:
a) providing a gelatine extract or a series of gelatine extracts, differing in bloom and viscosity,
b) subjecting a portion of one or more of the gelatine extracts of step a) to an enzymatic treatment to obtain one or more enzymatically treated extracts having a viscosity that is lower than that of the respective extract before said enzymatic treatment, while maintaining the bloom substantially unaffected, the enzymatically treated extracts comprising less than 1.5 w/w % of gelatine-derived peptides having a molecular weight of less than 2000 Da, wherein said enzymatic treatment is carried out using one or more endoproteases,
c) selecting one or more gelatine extracts of step a),
d) combine the selected one or more gelatine extracts of step c) and blend with one or more enzymatically treated extracts of step b) to provide a gelatine composition having a lower viscosity as compared to that of the said combined selected extracts of step c) without being blended with said selected one or more enzymatically treated gelatine extracts of step b), while maintaining the bloom substantially unaffected.

### Claim 2 (depends on 1)

The process according to claim 1, wherein step a) comprises the preparation of a series of extractions, wherein
i. a first extraction step results in a first gelatine extract having a first viscosity and a first bloom, followed by,
ii. a subsequent second extraction step resulting in a second additional gelatine extract having a second viscosity and a second bloom, optionally followed by,
iii.one or more subsequent additional extraction steps, each resulting in a subsequent additional gelatine extract having a subsequent viscosity and a subsequent bloom.

### Claim 3 (depends on 1)

The process according to claim 1, wherein at least one of the extracts of step a) has a viscosity higher than 3.0 mPa·s, wherein said viscosity is measured on a 6.67 wt. % aqueous solution at 60° C.

### Claim 4 (depends on 1)

The process according to claim 1 wherein the gelatine extract or a series of gelatine extracts of step a) is obtained by treatment of one or more gelatine-comprising raw materials under alkaline or acidic conditions.

### Claim 5 (depends on 4)

The process according to claim 4, wherein the one or more gelatine-comprising raw materials are obtained from one or more sources selected from the group consisting of bovine, porcine, fish, poultry, sheep and goat.

### Claim 6 (depends on 1)

The process according to claim 1, wherein the gelatine blend obtained in step d) has
a viscosity of between 2.0-3.5 mPa·s and a bloom of between 135-200 g, when the gelatine blend is obtained from one or more gelatine extracts derived from a gelatine-comprising raw material treated under alkaline conditions, or
a viscosity of between 1.8-3.3 mPa·s and a bloom of between 150-300 g, when the gelatine blend is obtained from one or more gelatine extracts derived from a gelatine-comprising raw material treated under acidic conditions,
or a viscosity of between 2.0-3.1 mPa·s and a bloom of between 180-220 g, when the gelatine blend is obtained from one or more gelatine extracts derived from a gelatine-comprising raw material treated under alkaline conditions and from a gelatine-comprising raw material treated under acidic conditions,
wherein the viscosity is measured on a 6.67 wt. % aqueous solution at 60° C.and the bloom on a 6.67 wt. % aqueous solution at 10° C.

### Claim 7 (depends on 1)

The process according to claim 1, further comprising the steps of:
e1) of measuring the pH of the gelatine blend of step d),
e2) adjusting the pH to be:
between 4.9-5.2, if the pH measured in step e1) is above 5.3, and when the gelatine blend is obtained from one or more gelatine extracts derived from a gelatine-comprising raw material treated under alkaline conditions,
between 4.3-5.5, if the pH measure in step e1) is above 5.6, and when the gelatine blend is obtained from one or more gelatine extracts derived from a gelatine-comprising raw material treated under acidic conditions,
between 4.5-5, if the pH measure in step e1) is
above 5.0, and when the gelatine blend is obtained from one or more gelatine extracts derived from a gelatine-comprising raw material treated under alkaline conditions and a gelatine-comprising raw material treated under acidic conditions.

### Claim 8 (depends on 7)

The process according to claim 7, wherein said process further comprises the step of:
f) adding between 0.01 and 10 wt .-% ammonium salt, based on the total weight of the composition, to the gelatine blend of step d), e1) or e2).

### Claim 9 (depends on 1)

A gelatine composition obtainable by the method of claim 1,
wherein the gelatine composition has an increased crosslinking time as measured by the time point where the viscosity value is higher than 60,000 mPa·s in a 20% gelatine solution in water after addition of 0.4 w/w % formaldehyde to the gelatine solution at 50° C.

### Claim 10 (depends on 9)

The gelatine composition according to claim 9, comprising an alkaline gelatine having a bloom of between 135-200 g, and a viscosity of between 2.0-3.5 mPa·s, wherein the viscosity is measured on a 6.67 wt. % aqueous solution at 60° C., and the bloom is measured on a 6.67 wt. % aqueous solution at 10° C., wherein said gelatine composition has a pH of between 4.9-5.2; and wherein said gelatine composition comprises less than 1.5 wt. % of free amino acids and peptides having a molecular weight of less than 2000 Da.

### Claim 11 (depends on 9)

The gelatine composition according to claim 9, comprising an acid gelatine having a bloom of between 150-300 g, and a viscosity of between 1.8-3.3 mPa·s, wherein the viscosity is measured on a 6.67 wt. % aqueous solution at 60° C., and the bloom is measured on a 6.67 wt. % aqueous solution at 10° C., wherein said gelatine composition has a pH of between 4.3-5.5 and wherein said gelatine composition comprises less than 1.5 wt. % of free amino acids and peptides having a molecular weight of less than 2000 Da.

### Claim 12 (depends on 9)

The gelatine composition according to claim 9, comprising an alkaline and an acid gelatine having a bloom of between 180-220 g, and a viscosity of between 2.0-3.1 mPa·s, wherein the viscosity is measured on a 6.67 wt. % aqueous solution at 60° C., and the bloom is measured on a 6.67 wt. % aqueous solution at 10° C., wherein said gelatine composition has a pH of between 4.5-5.0; and wherein said gelatine composition comprises less than 1.5 wt. % of free amino acids and peptides having a molecular weight of less than 2000 Da.

### Claim 13 (depends on 9)

The gelatine composition according to claim 9, wherein said gelatine composition further comprises between 0.01-10 wt. % ammonium salt based on the total weight of the gelatine composition.

### Claim 14 (depends on 9)

The gelatine composition according to claim 9, wherein said gelatine composition has an average molecular weight of between 50,000-200,000 Da.

### Claim 15 (depends on 10)

The gelatine composition according to claim 10, wherein said gelatine composition is obtained by a process for the preparation of a gelatine blend comprising the steps of:
a) providing a gelatine extract or a series of gelatine extracts, differing in bloom and viscosity,
b) subjecting a portion of one or more of the gelatine extracts of step a) to an enzymatic treatment to obtain one or more enzymatically treated extracts having a viscosity that is lower than that of the respective extract before said enzymatic treatment, while maintaining the bloom substantially unaffected, the enzymatically treated extracts comprising less than 1.5 w/w % of gelatine-derived peptides having a molecular weight of less than 2000 Da,
c) selecting one or more gelatine extracts of step a),
d) combine the selected one or more gelatine extracts of step c) and blend with one or more enzymatically treated extracts of step b) to provide a gelatine composition having a lower viscosity as compared to that of the said combined selected extracts of step c) without being blended with said selected one or more enzymatically treated gelatine extracts of step b), while maintaining the bloom substantially unaffected.

### Claim 16 (depends on 11)

The gelatine composition according to claim 11, wherein said gelatine composition is obtained by a process for the preparation of a gelatine blend comprising the steps of:
a) providing a gelatine extract or a series of gelatine extracts, differing in bloom and viscosity,
b) subjecting a portion of one or more of the gelatine extracts of step a) to an enzymatic treatment to obtain one or more enzymatically treated extracts having a viscosity that is lower than that of the respective extract before said enzymatic treatment, while maintaining the bloom substantially unaffected, the enzymatically treated extracts comprising less than 1.5 w/w % of gelatine-derived peptides having a molecular weight of less than 2000 Da,
c) selecting one or more gelatine extracts of step a),
d) combine the selected one or more gelatine extracts of step c) and blend with one or more enzymatically treated extracts of step b) to provide a gelatine composition having a lower viscosity as compared to that of the said combined selected extracts of step c) without being blended with said selected one or more enzymatically treated gelatine extracts of step b), while maintaining the bloom substantially unaffected.

### Claim 17 (depends on 12)

The gelatine composition according to claim 12, wherein said gelatine wherein said gelatine composition is obtained by a process for the preparation of a gelatine blend comprising the steps of:
a) providing a gelatine extract or a series of gelatine extracts, differing in bloom and viscosity,
b) subjecting a portion of one or more of the gelatine extracts of step a) to an enzymatic treatment to obtain one or more enzymatically treated extracts having a viscosity that is lower than that of the respective extract before said enzymatic treatment, while maintaining the bloom substantially unaffected, the enzymatically treated extracts comprising less than 1.5 w/w% of gelatine-derived peptides having a molecular weight of less than 2000 Da,
c) selecting one or more gelatine extracts of step a),
d) combine the selected one or more gelatine extracts of step c) and blend with one or more enzymatically treated extracts of step b) to provide a gelatine composition having a lower viscosity as compared to that of the said combined selected extracts of step c) without being blended with said selected one or more enzymatically treated gelatine extracts of step b), while maintaining the bloom substantially unaffected.

### Claim 18 (depends on 9)

The gelatine product comprising a gelatine composition according to claim 9, said product being selected from the group consisting of film, capsule, casing or coating.

### Claim 19

A gelatine product comprising a gelatine composition obtainable by a process for the preparation of a gelatine blend comprising the steps of:
a) providing a gelatine extract or a series of gelatine extracts, differing in bloom and viscosity,
b) subjecting a portion of one or more of the gelatine extracts of step a) to an enzymatic treatment to obtain one or more enzymatically treated extracts having a viscosity that is lower than that of the respective extract before said enzymatic treatment, while maintaining the bloom substantially unaffected, the enzymatically treated extracts comprising less than 1.5 w/w % of gelatine-derived peptides having a molecular weight of less than 2000 Da, wherein said enzymatic treatment is carried out using one or more endoproteases,
c) selecting one or more gelatine extracts of step a),
d) combine the selected one or more gelatine extracts of step c) and blend with one or more enzymatically treated extracts of step b) to provide a gelatine composition having a lower viscosity as compared to that of the said combined selected extracts of step c) without being blended with said selected one or more enzymatically treated gelatine extracts of step b), while maintaining the bloom substantially unaffected,
wherein the gelatine composition has an increased crosslinking time as measured by the time point where the viscosity value is higher than 60,000 mPa·s in a 20% gelatine solution in water after addition of 0.4 w/w % formaldehyde to the gelatine solution at 50° C.