Back to results
US 11,795,483 B2Granted
Self-assembled nanoplatelet-enzyme bioconjugates providing for increased biocatalytic efficiency
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorsIgor L. Medintz, Joyce Breger, Scott Walper, Michael H. Stewart
Life of this patent
Estimating the term…
Abstract
A nanoplatelet serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes or enzymes immobilized to quantum dots.
Claims6
A method of conducting a cascade reaction, comprising:
- providing a cascade cluster comprising a plurality of nanoplatelets associated together as a cluster, wherein each nanoplatelet is bound to a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth, wherein the enzymatic cascade comprises at least two different enzymes, and wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter;
- contacting the cascade cluster with a substrate of the first enzyme;
- and
- allowing a reaction to proceed so that each of the plurality of enzymes acts in succession to produce an end product,
- wherein the reaction is performed without stirring or mixing.
2 more elements
A method of preparing an enzymatic cascade cluster, the method comprising:
- contacting a plurality of nanoplatelets with a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth, wherein the enzymatic cascade comprises at least two different enzymes;
- thereby forming an enzymatic cascade cluster wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter,
- wherein at least one of the enzymes comprises multiple (His)6 tags effective to cross-link the nanoplatelets into the cluster.
Dependencies are inferred from the claim text.