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US 11,795,455 B2Granted
RNA cleavage-induced transcript stabilizer and uses thereof
AssigneeMassachusetts Institute of Technology
InventorsRon Weiss, Breanna E. DiAndreth
Life of this patent
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Abstract
Provided herein are genetic circuits and encoded RNA transcripts that produce an output molecule in response to an RNA cleavage event that removes a degradation signal. In some embodiments, the genetic circuits described herein may be used for detecting RNA cleaver activities (e.g., in a cell). Methods of using the genetic circuits described herein in diagnostic or therapeutic applications are also provided.
Claims14
A composition comprising:
- (i) a first genetic circuit encoding an RNA transcript, the genetic circuit comprising
- a first promoter operably linked to a nucleotide sequence encoding an output molecule followed, from 5′ to 3′, by an RNA stabilizer, a cleavage site for an RNA cleaver, and a degradation signal, wherein the RNA transcript comprises one or more recognition sites for an RNA repressor operably linked to the nucleotide sequence encoding the output molecule;
- and
- (ii) a second genetic circuit comprising a second promoter operably linked to a nucleotide sequence encoding the RNA repressor, and one or more cleavage sites for the RNA cleaver.
1 more element
Dependencies are inferred from the claim text.