Cultured phagocyte supernatent compositions
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Etablissement Francais Du Sang, Universite De Franche-Comte, Centre Hospitalier Regional Universitaire Besanc̨On
InventorsSylvain Perruche, Philippe Saas, Francis Bonnefoy
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Abstract
A composition including a supernatant obtained from co-culture of phagocytes with apoptotic cells. The composition is obtained by a) providing phagocytes, b) providing apoptotic cells, c) optionally washing the cells from step a) and b), d) co-culturing the cells of step a) and b), and e) separating the supernatant from the cells. The composition may be used in preventing or treating a pathological immune response.
Claims11
A composition comprising a supernatant, the supernatant obtained by:
- a) providing macrophages;
- b) providing apoptotic cells;
- c) optionally washing the cells from step a) and b);
- d) co-culturing the cells of step a) and b) for hours or days;
- and
- e) separating the supernatant from the cells.
A composition comprising a supernatant, the supernatant obtained by:
- a) providing dendritic cells;
- b) providing apoptotic cells;
- c) optionally washing the cells from step a) and b);
- d) co-culturing the cells of step a) and b) for hours or days;
- and
- e) separating the supernatant from the cells.
A composition comprising a supernatant, the supernatant obtained by:
- a) providing an amount of viable phagocytes, the viable phagocytes being phagocytes that have not been subjected to an apoptosis induction;
- b) providing apoptotic cells;
- c) optionally washing the cells from step a) and b);
- d) co-culturing the cells of step a) and b) until anti-inflammatory compounds are present;
- and
- e) separating the supernatant, which includes the anti-inflammatory compounds, from the cells.
Dependencies are inferred from the claim text.