# US 11,793,204 B2 · BVP8 protein for killing Tetranychid mites and use thereof

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- Number: US 11,793,204 B2
- Document ID: US11793204B2
- Status: Granted
- Kind: B2
- Application number: 17/832,566
- Filed: 2022-06-03
- Published: 2023-01-12
- Granted: 2023-10-24
- Earliest claimed: 2021-07-05
- Assignees: Hubei Biopesticide Engineering Research Center
- Applicants: Hubei Biopesticide Engineering Research Center
- Inventors: Xiaoyan Liu; Yong Min; Ling Chen; Lei Zhu; Yimin Qiu; Ben Rao; Ronghua Zhou; Yan Gong; Xianqing Liao; Wei Chen; Chunfu Qiu; Liqiao Shi; Jingzhong Yang
- Web page: https://patentgrep.com/patent/US11793204B2

## CPC

- A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46)
- A01P 7/02: Acaricides
- C07K 14/005: from viruses
- C12N 2710/16722: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2710/16731: Uses of virus other than therapeutic or vaccine, e.g. disinfectant
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A BVP8 protein for killing tetranychid mites and use thereof are provided. The protein is as set forth in SEQ ID NO. 2. The BVP8 protein has a median lethal concentration of 12.98 μg/mL against Tetranychus urticae, 33.45 μg/mL against Panonychus citri, and 26.32 μg/mL against Tetranychus cinnabarinus, and shows an inhibitory effect against the hatching and cleavage of Tetranychus urticae eggs, with the egg cleavage rate of 75.86% after 72 h. The protein provides a new option for the preparation of a novel miticide.

## Claims

### Claim 1

A method for controlling tetranychid mites, comprising
spraying a biocide comprising a protein of SEQ ID NO:2 on tetranychid mites.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the tetranychid mites are Tetranychus urticae, Panonychus citri, or Tetranychus cinnabarinus.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the protein of SEQ ID NO:2 has a median lethal concentration of 12.98 μg/mL against Tetranychus urticae.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the protein of SEQ ID NO:2 has a median lethal concentration of 33.45 μg/mL against Panonychus citri.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the protein of SEQ ID NO:2 has a median lethal concentration of 26.32 μg/mL against Tetranychus cinnabarinus.

### Claim 6

A method for inhibiting hatching of tetranychid mite eggs, comprising
spraying a biocide containing a protein as set forth in SEQ ID NO:2 to eggs of tetranychid mites.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the tetranychid mites are Tetranychus urticae, Panonychus citri, or Tetranychus cinnabarinus.

### Claim 8 (depends on 6)

The method according to claim 6, wherein a cleavage rate is 75.86% or less at 72 hours after spraying the biocide for Tetranychus urticae eggs.