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US 11,789,011 B2Granted
Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model
OwnerShelby Marie King, Deborah Lynn Greene Nguyen +6inventors
InventorsShelby Marie King, Deborah Lynn Greene Nguyen, Vivian A. Gorgen, Benjamin R. Shepherd, Sharon C. Presnell, Rosalie Sears, Brittany Allen-Petersen, Ellen Langer
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Abstract
Described are three-dimensional, engineered, biological breast tissues, adipose tissues, and tumor models, including breast cancer models.
Claims23
A culture comprising a three-dimensional, engineered, biological breast cancer model and a cell culture media,
- wherein the breast cancer model comprises:
- a. breast stromal tissue comprising human mammary fibroblasts, human endothelial cells, and human mesenchymal stem cells;
- and
- b. breast cancer tumor tissue comprising breast cancer cells and human endothelial cells, the tumor tissue surrounded on all sides by the stromal tissue to form the three-dimensional, engineered, biological breast cancer model;
- wherein the cell culture media comprises an adipocyte differentiation signal for differentiation of the human mesenchymal stem cells in the breast cancer model into human adipocytes, and
- wherein the human mesenchymal stem cells in the breast cancer model that are exposed to the adipocyte differentiation signal are capable of differentiating into adipocytes in the culture.
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An array of cultures, wherein each culture in the array comprises a three-dimensional, engineered, biological breast cancer model and a cell culture media,
- wherein the breast cancer model comprises:
- a. breast stromal tissue comprising human mammary fibroblasts, human endothelial cells, and human mesenchymal stem cells;
- and
- b. breast cancer tumor tissue comprising breast cancer cells and human endothelial cells, the tumor tissue surrounded on all sides by the stromal tissue to form each three-dimensional, engineered, biological breast cancer model;
- wherein the cell culture media comprises an adipocyte differentiation signal for differentiation of the human mesenchymal stem cells in the breast cancer model into human adipocytes,
- wherein the human mesenchymal stem cells in the breast cancer model that are exposed to the adipocyte differentiation signal are capable of differentiating into adipocytes in each culture, and wherein the array is adapted for use in a high throughput assay.
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Dependencies are inferred from the claim text.