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US 11,788,109 B2Granted
Microorganism and method for producing gamma-glutamyl-valyl-glycine
AssigneeAjinomoto Co., Inc.
InventorsAyako Sasahara, Takayuki Ito, Hiroyuki Nozaki
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Abstract
A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDIQ is reduced as an expression host, γ-Glu-Val-Gly is produced (Yom Glu, Val, and Gly as raw materials.
Claims14
A modified, Escherichia coli bacterium,
- wherein the bacterium has a deleted ybdK gene that encodes γ-glutamylglycine synthetase that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 16, wherein the bacterium has a reduced γ-glutamylglycine synthetase activity compared to a non-modified bacterium,
- wherein the bacterium has a deleted gshA gene that encodes a γ-glutamylcysteine synthetase that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 24,
- wherein the bacterium has a reduced γ-glutamylcysteine synthetase activity compared to a non-modified bacterium,
- wherein the bacterium has a deleted gene that encodes a γ-glutamyltransferase that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 26, wherein the bacterium has a reduced γ-glutamyltransferase activity compared to a non-modified bacterium,
- wherein the bacterium has a gene encoding γ-glutamylvaline synthetase, wherein the γ-glutamylvaline synthetase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 24 mutated with a substitution at a position selected from L135, Q144, Y241, N243, and Y300, or wherein the γ-glutamylvaline synthetase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 18, 20, or 22,
- wherein the γ-glutamylvaline synthetase has a ratio of γ-glutamylvaline synthetase activity to γ-glutamylglycine synthetase activity of at least 3.0, and
- wherein the bacterium has enhanced ability to produce γ-glutamylvaline or γ-glutamylvalylglycine compared to a non-modified bacterium.
9 more elements
Dependencies are inferred from the claim text.