CRISPR/Cas system and method for genome editing and modulating transcription
AssigneeThe Board of Trustees of the University of Illinois
InventorsRyan E. Clarke, Bradley J. Merrill, Matthew S. MacDougall, Hannah M. Pennington, Brian R. Shy
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Abstract
A CRISPR/Cas system and method for editing or regulating transcription of a genome of a cell are provided, wherein the system includes a Cas endonuclease fused with one or more degron sequences and at least one activatable cognate single guide RNA harboring an inactivation sequence in a non-essential region of the cognate sgRNA, wherein said inactivation sequence comprises one or more endonuclease recognition sites of, e.g., a ribozyme.
Claims8
A CRISPR/Cas system comprising a Cas endonuclease and a cognate single guide RNA (sgRNA), wherein the cognate sgRNA is an activatable sgRNA harboring an inactivation sequence in a non-essential region of the sgRNA, wherein said inactivation sequence comprises one or more RNA-guided endonuclease recognition sites, and the activatable sgRNA is encoded by a nucleic acid molecule of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 or SEQ ID NO:22.
An activatable single guide RNA (sgRNA) harboring an inactivation sequence in a non-essential region of the sgRNA, wherein said inactivation sequence comprises one or more RNA-guided endonuclease recognition sites, and wherein the activatable sgRNA is encoded by a nucleic acid molecule of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 or SEQ ID NO:22.
A CRISPR/Cas system comprising a Cas endonuclease and a cognate single guide RNA (sgRNA), wherein the cognate sgRNA is an activatable sgRNA harboring an inactivation sequence in a non-essential region of the activatable sgRNA, wherein said inactivation sequence comprises one or more endonuclease recognition sites and the inactivation sequence is a cis-acting ribozyme encoded by a nucleic acid molecule of SEQ ID NO:2 or SEQ ID NO:3.
An activatable single guide RNA (sgRNA) harboring an inactivation sequence in a non-essential region of the sgRNA, wherein said inactivation sequence comprises one or more endonuclease recognition sites, and the inactivation sequence is a cis-acting ribozyme encoded by a nucleic acid molecule of SEQ ID NO:2 or SEQ ID NO:3.
Dependencies are inferred from the claim text.