Antibody conjugates and methods of making and using the same
AssigneeR.P. Scherer Technologies, LLC
InventorsYun Cheol Kim, Chao Bai Huang, David Rabuka
Life of this patent
Estimating the term…
Abstract
Antibodies that include a sulfatase motif-containing tag in a constant region of an immunoglobulin (Ig) light chain polypeptide are disclosed. The sulfatase motif can be converted by a formylglycine-generating enzyme (FGE) to produce a formylglycine (fGly)-modified Ig light chain polypeptide. An fGly-modified Ig light chain polypeptide of the antibody can be covalently and site-specifically bound to a moiety of interest to provide an antibody conjugate. The disclosure also encompasses methods of production of such tagged Ig light chain polypeptides, fGly-modified Ig light chain polypeptides, and antibody conjugates, as well as methods of use of same.
Claims29
An immunoglobulin (Ig) kappa light chain polypeptide comprising, in a constant region, an amino acid sequence selected from:
- i) SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 26, SEQ ID NO:
- 28, SEQ ID NO:
- 31, SEQ ID NO:
- 37, SEQ ID NO:
- 44, and SEQ ID NO:
- 45;
- wherein the cysteine residue in the sequence LCTPSR (SEQ ID NO:
- 158) is oxidized to fGly.
Dependencies are inferred from the claim text.