Systems and methods for a collapsible chamber with foldable mixing element
AssigneeLife Technologies Corporation
InventorStephan Kaiser
Life of this patent
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Abstract
The present set of embodiments relate to a system, method, and apparatus for culturing cells within a cell culture vessel having a mixing element. The cell culture system includes a flexible portion and a mixing element disposed therein. The mixing element includes a suspended foldable portion. The system is configured to reduce shear stress on cells without compromising mixing efficiency. This reduction is accomplished by using a mixing element having a large surface area allowing for reduced rotational speeds. The system is collapsible for ease of transport and disposal. The flexible portion collapses and the foldable portion folds to minimize the volume of the system while not in operation.
Claims17
A collapsible cell culture vessel, comprising:
- a flexible portion comprising a collapsible container bounding a chamber that is adapted to support a cell culture;
- and
- a mixing element disposed within the collapsible container, the mixing element comprising:
- a first suspension element rotatably disposed within the chamber of the collapsible container;
- a second suspension element rotatably disposed within the chamber of the collapsible container, the second suspension element being spaced apart from the first suspension element;
- one or more foldable portions each extending between a first end and an opposing second end, the first end being connected to the first suspension element and the second end being connected to the second suspension element, each of the one or more foldable portions comprising a flexible sheet having a front face and an opposing back face that both extend between the first end and the opposing second end;
- and
- a surface feature outwardly projecting from the front face of the flexible sheet of each of the one or more foldable portions.
A collapsible cell culture vessel, comprising:
- a collapsible container bounding a chamber adapted to support a cell culture;
- and
- a mixing element disposed within the collapsible container, the mixing element comprising:
- a first suspension element disposed within the chamber of the collapsible container, the first suspension element comprising a first hub rotatably disposed within the chamber of the collapsible container and a first arm projecting from the first hub;
- a second suspension element disposed within the chamber of the collapsible container, the second suspension element comprising a second hub rotatably disposed within the chamber of the collapsible container and a first arm projecting from the second hub;
- one or more foldable portions each extending between the first suspension element and the second suspension element and each comprising a flexible sheet having a front face and an opposing back face, the one or more foldable portions comprising a first flexible sheet having the front face and the opposing back face that extend between the first arm projecting from the first hub and the first arm projecting from the second hub, the first flexible sheet being sufficiently flexible that it can be folded over an angle between about 90 degrees and about 179 degrees;
- and
- a surface feature outwardly projecting from the front face of the flexible sheet of each of the one or more foldable portions.
A method of culturing cells, comprising:
- delivering a cell culture into a collapsible container;
- and
- mixing the cell culture within collapsible container using a mixing element disposed within the collapsible container, the mixing element comprising:
- a first suspension element rotatably disposed within the collapsible container;
- a second suspension element rotatably disposed within the collapsible container;
- a first foldable portion extending between a first end and an opposing second end, the first end being connected to the first suspension element and the second end being connected to the second suspension element, the first foldable portion comprising a flexible first sheet having a front face and an opposing back face that both extend between the first end and the opposing second end;
- and
- a surface feature outwardly projecting from the front face of the flexible first sheet.
Dependencies are inferred from the claim text.