Polylactide cell culture containers and use in cell culture
AssigneeDiversified Biotech, Inc.
InventorDaniel Perlman
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Abstract
2D and 3D cell culture containers formed from blends of poly-L-lactide and poly-D-lactide provide growth surfaces for adherent cells and do not require surface treatment or coating to support mammalian cell growth. The cell culture containers are transparent, heat tolerant, and are environmentally degradable and suitable for composting in landfills.
Claims20
A cell culture container consisting essentially of a polylactide polymer material, the material consisting of (100−X) wt % of poly-L-lactide and X wt % of poly-D-lactide, wherein X≤10, wherein 100 wt % represents the total weight of polylactide polymers in the material, and wherein the cell culture container is formed from the polymer material by a forced flow molding method and has been sterilized by gamma irradiation or electron beam irradiation, thereby increasing the hydrophilicity of a cell growth surface of the container compared to the cell growth surface without said irradiation.
A cell growth structure consisting essentially of a polylactide polymer material consisting of (100−X) wt % of poly-L-lactide and X wt % of poly-D-lactide, wherein X≤10, and wherein the cell growth structure is formed by a forced flow molding method and has been sterilized by gamma irradiation or electron beam irradiation, thereby increasing the hydrophilicity of a cell growth surface of the cell growth structure compared to the cell growth surface without said irradiation.
A method of making a cell culture container, the method comprising:
- (a) heating a polylactide polymer material consisting of (100−X) wt % of poly-L-lactide and X wt % of poly-D-lactide, wherein X≤10 to a molding temperature, and wherein 100 wt % represents the total weight of polylactide polymers in the mixture;
- (b) subjecting the material to a molding method comprising forced flow molding to form a structure having a shape of said cell culture container;
- (c) cooling the structure;
- (d) optionally annealing the structure for an annealing time at an annealing temperature;
- and
- (e) sterilizing the container obtained from step (c) or step (d) with gamma irradiation or electron beam irradiation;
- whereby said cell culture container is obtained.
Dependencies are inferred from the claim text.