Continuous glucose monitoring using an FAD-dependent glucose dehydrogenase

A method of continuous glucose monitoring comprising using a glucose dehydrogenase (GDH) capable of

• (a) retaining 60% or more of the initial activity over a period of 1 week when retained at 40° C. in solution when retained at a pH of 7.4,
• (b) retaining 40% or more of the initial activity over a period of 2 weeks when retained at 40° C. in solution when retained at a pH of 7.4, or
• (c) retaining 30% or more of the initial activity over a period of 3 weeks when retained at 40° C. in solution when retained at a pH of 7.4,
• wherein said GDH is a flavin adenine dinucleotide (FAD) dependent GDH and wherein said GDH is neither a membrane bound protein nor GLD3,
• wherein the continuous glucose monitoring comprises
• i) bringing a sample containing glucose into contact with a glucose sensor, wherein the glucose sensor comprises an electrode, the GDH and a mediator, wherein the GDH is immobilized on the electrode and wherein the mediator is immobilized on the electrode,
• ii) applying a potential to the electrode, and
• iii) measuring the response current,
• wherein the continuous glucose monitoring is carried out for 1 day or more.