Reducing the accumulation of imines/enamines for the production of amino acids or amino acid-derived products
AssigneesCathay Biotech Inc., Cibt America Inc.
InventorsHoward Chou, Ling Chen, Xiucai Liu
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Abstract
Provided microorganisms genetically modified to overexpress an imine/enamine deaminase to enhance the production of lysine and lysine derivatives by the microorganism. Also provided a method of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.
Claims14
A genetically modified host cell that enhances removal of imine and enamine compounds, said host cell comprising an exogenous polynucleotide that encodes and overexpresses an imine/enamine deaminase polypeptide that increases the amount of an amino acid or amino acid derivative compared to a host cell that has not been modified to express the exogenous polynucleotide, wherein the host cell is of the genus Escherichia, the amino acid is lysine, and the amino acid derivative is cadaverine, and wherein the imine/enamine deaminase polypeptide:
- (i) has at least 95% identity to the amino acid sequence of SEQ ID NO:
- 12;
- or
- (ii) comprises the amino acid sequence of SEQ ID NO:
- 12.
Dependencies are inferred from the claim text.