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US 11,781,122 B2Granted
Ectoine-producing yeast
AssigneeGivaudan SA
InventorsDominique Louis, Karine Jaillardon, Dominique Thomas
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Abstract
The present invention relates to the field of bio-production of ectoine. There is a need in the art for ectoine production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified yeast comprising many modifications as described in the present text.
Claims15
An ectoine-producing recombinant yeast, in the genome of which:
- (A) (i) at least one nucleic acid encoding an aspartokinase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and/or
- (ii) at least one nucleic acid encoding an aspartate kinase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (B) at least one nucleic acid encoding an aspartate semi-aldehyde dehydrogenase that can use as coenzyme both NAD and NADP is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (C) at least one nucleic acid encoding a diaminobutyrate aminotransferase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (D) (i) at least one nucleic acid encoding an homoserine-O-acetyltransferase METX is overexpressed and/or is under the control of an inducible or repressible promoter that is heterologous from the nucleic acid encoding METX, and
- (ii) at least one nucleic acid encoding a diaminobutyric acid acetyltransferase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (E) at least one nucleic acid encoding an ectoine synthase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (F) (i) at least one endogenous nucleic acid encoding an homoserine dehydrogenase has been deleted and/or interrupted, and/or
- (ii) at least one nucleic acid encoding a homoserine dehydrogenase is:
- under the control of an inducible or repressible promoter;
- under the control of a weak promoter;
- and/or
- the homoserine dehydrogenase is degron-tagged.
16 more elements
Dependencies are inferred from the claim text.