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US 11,780,895 B2Granted
Targeted DNA demethylation and methylation
AssigneeThe Jackson Laboratory
InventorsAlbert Cheng, Aziz Taghbalout, Nathaniel Jillette
Life of this patent
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Abstract
Provided herein are, inter alia, compositions and methods for demethylating and methylating a target DNA sequences in a mammalian cell. The compositions and methods are, useful for activity modulation of a targeted gene, or to create a gene regulatory network.
Claims22
A composition comprising:
- a guide RNA (gRNA) comprising a PUF binding site (PBS) sequence;
- a nuclease-deficient DNA endonuclease that binds to the gRNA;
- and
- a PUF domain linked to a demethylation domain or a methylation domain, wherein the PUF domain binds to the PBS of the gRNA.
1 more element
A kit comprising:
- a guide RNA (gRNA) comprising a PUF binding site (PBS) sequence;
- a nuclease-deficient DNA endonuclease that binds to the gRNA;
- and a PUF domain linked to a demethylation domain or a methylation domain, wherein the PUF domain binds to the PBS of the gRNA.
A complex comprising:
- a guide RNA (gRNA) comprising a PUF binding site (PBS) sequence;
- and a protein comprising a PUF domain linked to a demethylation domain or a methylation domain, wherein the PUF domain is bound to the PBS of the gRNA.
Dependencies are inferred from the claim text.