Quinone methide analog signal amplification

A compound, having formula (III):

• wherein
• each Q is Carbon;
• each R7 is independently selected from hydrogen, —Z—R1, a moiety comprising a LG group, —C(LG)(R5)(R3R4), —R3R4, a moiety comprising a LG group and a detectable label, a halo group, an aliphatic group, an alkoxy group, or where two adjacent R7 groups together form an aliphatic ring or aryl ring where the aliphatic ring or aryl ring formed from the two adjacent R7 groups is substituted with at least an LG group and a moiety comprising a detectable label;
• —Z—R1 is —OP(O)(OH)2, a phosphate ester, or —O-sugar;
• LG is a halide, alkoxy, carboxylate, inorganic ester, thiolate, amine, carboxylate, azide, sulfate ester, aryloxy, phenoxide or —N(Rb)3+ where each Rb is independently hydrogen or a lower alkyl or two Rb moieties together form a heteroaliphatic ring;
• R3 is independently —(CH2)nNH—, —O(CH2)nNH—, —N(H)C(O)(CH2)nNH—, —C(O)N(H)(CH2)nNH—, —(CH2)nO—, —O(CH2)nO—, —O(CH2CH2O)n—, —N(H)C(O)(CH2)nO—, —C(O)N(H)(CH2)nO—, C(O)N(H)(CH2CH2O)n—, —(CH2)nS—, —O(CH2)nS—, —N(H)C(O)(CH2)nS—, —C(O)N(H)(CH2)nS—, —(CH2)nNH—, —C(O)N(H)(CH2CH2O)nCH2CH2NH—, —C(O)(CH2CH2O)nCH2CH2NH—, —C(O)N(H)(CH2)nNHC(O)CH(CH3)(CH2)nNH—, or —N(H)(CH2)nNH—, where each n independently is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12;
• m is 1,
• at least one of the R7 groups comprises —Z—R1;
• R4 is a detectable label selected from a hapten, an annulated chromophore, a chromogen capable of being visualized using bright-field illumination, a diazo dye, a diazo-containing chromogen, biotin, fluorescein, fluorescein derivatives, a coumarin, a coumarin derivative, a tartrazine, or a triarylmethane;
• R5 is H;
• wherein the compound of Formula (III) comprises at least one detectable label;
• and
• wherein the compound of Formula (III) comprises at least two adjacent R7 groups, wherein the at least two adjacent R7 groups comprise an indoline group.