Method for the cytometric analysis of cell samples

A method for a cytometric analysis of multiple cell samples by means of a microscope for examining multiple cell samples under a microscope, wherein the microscope is operated in a transmission mode and in a fluorescence mode, and wherein at least one cell sample has at least one fluorescence marker, wherein the method comprising:

• continuously moving the cell samples in an X-Y plane relative to an optical system of the microscope having at least one microscope camera, wherein, during the movement of the cell samples, at least one or more images of a sub-region of the cell samples are recorded in the transmission mode and at least one or more images of the same sub-region of the cell samples are recorded in the fluorescence mode by means of the at least one microscope camera, wherein the at least one or more images of the same sub-region of the cell samples in the transmission mode, and at least one or more images of the same sub-region of the cell samples in the fluorescence mode are associated with each other, and one or more of the following:
• (a) wherein the positions and/or contours of cells or cell components of the cell samples are detected in the at least one or more images of the transmission mode, and the detected intensities of the at least one or more images recorded in the fluorescence mode are then analyzed as a function of the positions and/or contours of cells or cell components in the cell samples detected in the transmission mode, and
• (b) wherein the positions and/or contours of cells or cell components of the cell samples are detected in the at least one or more images of the fluorescence mode, and the detected intensities of the at least one or more images recorded in the transmission mode are then analyzed as a function of the positions and/or contours of cells or cell components in the cell samples detected in the fluorescence mode,
• wherein at least one image of the sub-region of the cell samples in the transmission mode is recorded in a first Z-plane, and at least one image of the sub-region of the cell samples in the transmission mode is recorded in a second Z-plane, which is different from the first Z-plane, and wherein the second Z-plane is a defocused plane,
• an image position is adjusted by autofocus in a Z-plane while the cell samples are continuously moved in the X-Y plane in the transmission mode;
• the light passing through the cell samples in the transmission mode is detected by an objective and a digital camera, and
• a further optical element is provided between the objective and the digital camera in the beam path of the light in transmission mode in order to record images in two parallel Z-planes of the cell samples.

A method for a cytometric analysis of multiple cell samples by means of a microscope for examining multiple cell samples under a microscope, wherein the microscope is operated in a transmission mode and in a fluorescence mode, and wherein at least one cell sample has at least one fluorescence marker, wherein the method comprising:

• continuously moving the cell samples in one plane relative to an optical system of the microscope having at least one microscope camera, wherein, during the movement of the cell samples, at least one or more images of a sub-region of the cell samples are recorded in the transmission mode and at least one or more images of the same sub-region of the cell samples are recorded in the fluorescence mode by means of the at least one microscope camera, wherein the at least one or more images of the same sub-region of the cell samples in the transmission mode, and at least one or more images of the same sub-region of the cell samples in the fluorescence mode are associated with each other, and one or more of the following:
• (a) wherein the positions and/or contours of cells or cell components of the cell samples are detected in the at least one or more images of the transmission mode, and the detected intensities of the at least one or more images recorded in the fluorescence mode are then analyzed as a function of the positions and/or contours of cells or cell components in the cell samples detected in the transmission mode, and
• (b) wherein the positions and/or contours of cells or cell components of the cell samples are detected in the at least one or more images of the fluorescence mode, and the detected intensities of the at least one or more images recorded in the transmission mode are then analyzed as a function of the positions and/or contours of cells or cell components in the cell samples detected in the fluorescence mode,
• wherein at least one image of the sub-region of the cell samples in the transmission mode is recorded in a first plane, and at least one image of the sub-region of the cell samples in the transmission mode is recorded in a second plane, which is different from the first plane, and wherein the second plane is a defocused plane,
• further comprising generating phase-contrast images from the defocused transmission images using phase contrast by aberrations due to defocusing.