Iron chelator-containing prothrombin time reagent
AssigneeSiemens Healthcare Diagnostics Products GmbH
InventorsSabine Pilgrim, Thomas Wissel, Norbert Zander
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Abstract
The present invention pertains to the field of coagulation diagnostics and relates to a prothrombin time reagent on the basis of recombinant or native tissue factor protein and phospholipids, which reagent can be stabilized by the addition of an iron chelator from the groups of siderophores and 3,5-diphenyl-1,2,4-triazoles.
Claims16
A prothrombin time reagent comprising tissue factor protein and phospholipids, wherein the reagent further comprises at least one iron chelator, wherein the at least one iron chelator is a 3,5-diphenyl-1,2,4-triazole selected from the group consisting of deferasirox, ethyl [3,5-bis(2-hydroxyphenyl)-[1,2,4]triazol-1-yl]acetate, and 3,5-bis(2-hydroxyphenyl)-1-(2,2,2-trifluoroethyl)-1H-[1,2,4]triazole.
A method for preparing a liquid prothrombin time reagent comprising tissue factor protein and phospholipids, the method comprising filling a reagent vial with a liquid comprising tissue factor protein, phospholipids and at least one iron chelator without lyophilization, wherein the at least one iron chelator is a 3,5-diphenyl-1,2,4-triazole selected from the group consisting of deferasirox, ethyl [3,5-bis(2-hydroxyphenyl)-[1,2,4]triazol-1-yl]acetate, and 3,5-bis(2-hydroxyphenyl)-1-(2,2,2-trifluoroethyl)-1H-[1,2,4]triazole.
A method for stabilizing a prothrombin time reagent comprising tissue factor protein and phospholipids, the method comprising adding at least one iron chelator the reagent, wherein the at least one iron chelator is a 3,5-diphenyl-1,2,4-triazole selected from the group consisting of deferasirox, ethyl [3,5-bis(2-hydroxyphenyl)-[1,2,4]triazol-1-yl]acetate, and 3,5-bis(2-hydroxyphenyl)-1-(2,2,2-trifluoroethyl)-1H-[1,2,4]triazole.
Dependencies are inferred from the claim text.