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US 11,773,439 B2Granted
Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. Glezer, Andrew Spaventa, David Barker
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Abstract
The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.
Claims20
A method of sequencing a DNA molecule, comprising:
- (a) contacting a solid support comprising a primed template DNA molecule with a deoxyribonucleotide triphosphate (dNTP) analogue species, wherein said dNTP analogue species is complementary to a nucleotide of the primed template DNA molecule, and wherein said dNTP analogue species is associated with a detectable label;
- (b) after step (a), contacting said primed template DNA molecule with a plurality of blocked dNTP analogue species, and incorporating with a polymerase at least one of said plurality of blocked dNTP analogue species, thereby forming a blocked extension product;
- and
- (c) imaging said solid support during steps (a) and (b), wherein the combined steps (a)-(b) occurs for about 1 minute to about 10 minutes.
1 more element
Dependencies are inferred from the claim text.