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US 11,773,379 B2Granted
Enzymes and reagents for measurement of short chain fatty acids
AssigneeSysmex Corporation
InventorsShinichi Sakasegawa, Kenji Konishi, Yasushi Shirahase, Toshiyuki Yoshida
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Abstract
Disclosed are methods and reagents for the enzymatic measurement of short-chain fatty acids, having 3 to 6 carbon atoms, in a sample. Methods include the use of recombinant butyrate kinases from multiple species, combined various reagents that includes ATP, to detect butyric acid in different types of samples via measurement of ATP consumption. Disclosed also are the reagents themselves.
Claims9
A method for measuring the amount of a short-chain fatty acid having 3 to 6 carbon atoms in a sample, comprising:
- enzymatically reacting the short-chain fatty acid with adenosine triphosphate (ATP), and a butyrate kinase such that adenosine diphosphate (ADP) is produced, and
- measuring the amount of ADP produced;
- wherein said short-chain fatty acid is at least one selected from the group consisting of propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, and caproic acid,
- wherein the butyrate kinase is from Acetoanaerobium sticklandii or Thermosediminibacter oceani, and
- wherein the sample is at least one selected from the group consisting of saliva, gingival crevicular fluid, blood, plasma, serum and urine.
2 more elements
Dependencies are inferred from the claim text.