Method for amplifying CD8+T cells and cell subpopulations thereof in-vitro
AssigneeShanghai Innovative Chang'An Biological Technology Co., Ltd.
InventorsJianqing Xu, Xiaoyan Zhang, Chenli Qiu
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Abstract
Disclosed is a method for rapidly amplifying CD8+T cells and functional cell subpopulations thereof in vitro. A TLR1/2 agonist, a TLR2/6 agonist and a TLR5 agonist or a combination of above agonists is added to a conventional culture system for in-vitro amplification of CD8+T cells. Recombinant cytokines IL-2, IL-7 and IL-15 as well as magnetic beads coated with an anti-human CD3 antibody and an anti-human CD28 antibody can be further added to the culture system for continuous co-stimulation.
Claims7
A method for inducing in-vitro amplification of PD-1+CD8+T cell subpopulations, comprising adding anti-human CD3 antibody, anti-human CD28 antibody, cytokine IL-7, cytokine IL-15, and a Toll-like receptor agonist chosen from one or more in the group of TLR1/2 agonists, TLR2/6 agonists and TLR5 agonists, to a culture system for the PD-1+CD8+T cell subpopulations, thereby causing the in-vitro amplification and functional repair of the PD-1+CD8+T cell subpopulations thereof.
Dependencies are inferred from the claim text.