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US 11,773,371 B2Granted
Skin tissue
AssigneeUniversity of Durham
InventorsMartin Goldberg, Iakowos Karakesisoglou, James Carthew
Life of this patent
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Abstract
A method of creating skin tissue is described, particularly, an in vitro or ex vivo method for creating skin tissue. The invention extends to the use of agents that disrupt the LINC complex in a skin cell to create the skin tissue, and to using the created tissue in an assay to identify or screen anti-ageing compounds. The invention further extends to model skin tissues per se, uses thereof and to kits for creating such model skin tissues.
Claims11
A method of preparing skin tissue, the method comprising:
- (i) contacting a skin cell with an agent that disrupts the linker of the nucleoskeleton and cytoskeleton (LINC) complex of the cell, wherein the agent comprises:
- a. a modified Sad1 and UNC-84 (SUN) protein that consists of a SUN domain;
- or
- b. an amino acid sequence of SEQ ID No:
- 27;
- (ii) culturing the cell on a substrate comprising culture media to induce proliferation of the cell into a plurality of cells;
- and
- (iii) removing a portion of culture media from the substrate such that the plurality of cells are disposed in an interface between culture media remaining on the substrate and air, to thereby induce differentiation of the cells into skin tissue, wherein the skin cell of step (i) is a keratinocyte.
5 more elements
Dependencies are inferred from the claim text.