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US 11,773,134 B2Granted
Process of manufacture of annexin V
AssigneeAnnexin Pharmaceuticals AB
InventorsToomas Moks, Jan Christoph Reich
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AssigneeAnnexin Pharmaceuticals AB
InventorsToomas Moks, Jan Christoph Reich
Estimating the term…
The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.
A composition comprising a recombinant AnxA5 protein, wherein said AnxA5 protein differs from the sequence of human Annexin A5 (SEQ ID NO:1) at one or more positions by an amino acid insertion or deletion or substitution, wherein the AnxA5 protein is more than 95% identical to SEQ ID NO:
Dependencies are inferred from the claim text.