Back to results
US 11,772,063 B2Granted
Method and device for producing saccharides and saccharide arrays
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsFelix Löffler, Marco Mende, Stephan Eickelmann, Peter H. Seeberger, Alexandra Tsouka, Jasmin Heidepriem, Grigori Paris
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method and a device for producing saccharides and saccharide arrays. Said method is particularly useful for the synthesis of saccharides in parallel and of high-density saccharide arrays, such as microarrays, which are required for high-throughput screenings.
Claims15
A method for synthesizing saccharide comprising the steps:
- A) providing a solid support with at least one immobilized acceptor group for reacting with a saccharide;
- B) delivering the saccharide onto the solid support;
- C) applying a vapor of a mixture of a glycosylation reagent and a solvent onto the solid support at a temperature below 20° C. in order to initiate a coupling reaction of the saccharide to the at least one immobilized acceptor group.
A saccharide synthesizer comprising:
- a substrate, the substrate having a surface and being configured to support a solid support with at least one immobilized acceptor group for reacting with a saccharide;
- means for delivering a saccharide to a solid support with at least one immobilized acceptor group for reacting with a saccharide supported by the substrate;
- a chamber comprising
- a process space;
- a vapor supply in fluid communication with the process space, the vapor supply configured to supply a vapor comprising a solvent and a glycosylation reagent to the process space;
- a cooling element positioned within the processing chamber configured to cool the solid support by heat transfer through the substrate;
- an exhaust port in the processing chamber configured in fluid communication with an isolation valve;
- a purge gas supply in fluid communication with the process space, the purge gas supply configured to supply a purge gas to the process space effective to displace the vapor from the process space,
- wherein the substrate is positioned in the process space of the chamber.
6 more elements
Dependencies are inferred from the claim text.