Back to results
US 11,771,763 B2Granted
Methods and compositions for decreasing chronic pain
AssigneesEOS Neuroscience, Inc., Wake Forest University Health Sciences
InventorsAlan Horsager, Kenneth Greenberg, Benjamin C. Matteo, Edward S. Boyden, Douglas G. Ririe, James C. Eisenach, Christian Wentz
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions and methods for the selective silencing of neurons in pain pathway by using a combination of inhibitory light-sensitive protein gene transfer and wavelength specific illumination.
Claims19
A method to relieve neuropathic pain in a subject in need of such relief, comprising
- transfecting dorsal root ganglion (DRG) neurons of the subject with a polynucleotide using an adeno-associated viral (AAV) vector by intrathecal injection, wherein the polynucleotide comprises a regulatory region driving expression of a sequence encoding
- archaerhodopsin-3 (Arch) and the regulatory region comprises at least one of a preprotachykinin-A (PPT) promoter, a voltage-gated sodium channel subunit alpha (Scn10a) promoter, and a vanilloid receptor subtype 1 (TRPV1) promoter, and exposing Arch-expressing DRG neurons to light comprising a wavelength of from 495-570 nm, wherein the exposing Arch-expressing DRG neurons to light leads to a decrease in membrane potential relative to DRG neurons not expressing Arch.
Dependencies are inferred from the claim text.