Treatment and prevention of house dust mite allergies

A fusion protein having formula (I)

• X1−Y−X2 (I),
• wherein X1 and X2 comprise each four to eight allergen fragments or variants thereof fused to each other, wherein said allergen fragments are derived from at least two allergens of the genus Dermatophagoides, and wherein Y is a carrier protein,
• wherein the at least two allergens are of Dermatophagoides pteronyssinus and/or Dermatophagoides farina, and selected from the group consisting of Der p 1, Der p 2, Der p 5, Der p 7, Der p 21 and Der p 23, wherein the fusion protein comprises at least one polypeptide having amino acid sequence selected from the group consisting of SEQ ID No. 18, SEQ ID No. 19, SEQ ID No. 20, SEQ ID No. 21, SEQ ID No. 22, SEQ ID No. 23, SEQ ID No. 24 and/or SEQ ID No. 25, wherein
• a) the allergen fragment of Der p 1 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of TNACSINGNAPAEIDLRQMRTVTPIRMQGGCGSCWAFSGVA (SEQ ID No. 1), ATESAYLAYRNQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQ (SEQ ID No. 2), HNGVVQESYYRYVAREQSCRRPNAQRFGISN (SEQ ID No. 3), VRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL (SEQ ID No. 4), TNASSINGNAPAEIDLRQMRTVTPIRMQGGSGSSWAFSGVA (SEQ ID No. 5), ATESAYLAYRNQSLDLAEQELVDSASQHGSHGDTIPRGIEYIQ (SEQ ID No. 6) and HNGVVQESYYRYVAREQSSRRPNAQRFGISN (SEQ ID No. 7), and/or
• b) the allergen fragment of Der p 2 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of CHGSEPCIIHRGKPFQLEAVFEANQNSKTAK (SEQ ID No. 8), EVDVPGIDPNACHYMKCPLVKGQQYDIKYTWIVPKIAPKSEN (SEQ ID No. 9), HGSEPSIIHRGKPFQLEAVFEANQNSKTAK (SEQ ID No. 10) and EVDVPGIDPNASHYMKSPLVKGQQYDIKYTWIVPKIAPKSEN (SEQ ID No. 11), and/or
• c) the allergen fragment of Der p 5 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of DYQNEFDFLLMERIHEQIKKGELALFYLQ (SEQ ID No. 12) and EQYNLEMAKKSGDILERDLKKEEARVKKIEV (SEQ ID No. 13), and/or
• d) the allergen fragment of Der p 7 consists of amino acid sequence being at least 90% identical to amino acid sequence DPIHYDKITEEINKAVDEAVAAIEKSETFD (SEQ ID No. 14), and/or
• e) the allergen fragment of Der p 21 consists of amino acid sequence being at least 90% identical to amino acid sequence YNYEFALESIKLLIKKLDELAKKVKAVNPDEYY (SEQ ID No. 15), and/or
• f) the allergen fragment of Der p 23 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of GYFADPKDPHKFYICSNWEAVHKDCPGNTRWNEDEETCT (SEQ ID No. 16) and GYFADPKDPHKFYISSNWEAVHKDSPGNTRWNEDEETST (SEQ ID No. 17), and/or
• g) the allergen fragment of Der f 1 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of TSACRINSVNVPSELDLRSLRTVTPIRMQGGCGSCWAFSGVA (SEQ ID No. 38), ATESAYLAYRNTSLDLSEQELVDCASQHGCHGDTIPRGIEYIQ (SEQ ID No. 39), QNGVVEERSYPYVAREQQCRRPNSQHYGISN (SEQ ID No. 40) and VRNSWDTTWGDSGYGYFQAGNNLMMIEQYPYVVIM (SEQ ID No. 41), and/or
• h) the allergen fragment of Der f 2 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of HGSDPCIIHRGKPFNLEAIFDANQNTKTAK (SEQ ID No. 42) and EVDVPGIDTNACHYIKCPLVKGQQYDAKYTWNVPKIAPKSEN (SEQ ID No. 43), and/or
• i) the allergen fragment of Der f 5 consists of an amino acid sequence being at least 90% identical to an amino acid sequence selected from the group consisting of DYQNEFDFLLMQRIHEQMRKGEEALLHLQ (SEQ ID No. 44) and ERYNVEIALKSNEILERDLKKEEQRVKKIEV (SEQ ID No. 45), and/or
• j) the allergen fragment of Der f 7 consists of an amino acid sequence being at least 90% identical to amino acid sequence DPIHYDKITEEINKAIDDAIAAIEQSETID (SEQ ID No. 46), and/or
• k) the allergen fragment of Der f 21 consists of an amino acid sequence being at least 90% identical to amino acid sequence YNFETA VSTIEILVKDLAELAKKVKAVKSDD (SEQ ID No. 47), and/or
• l) the allergen fragment of Der f 23 consists of an amino acid sequence being at least 90% identical to amino acid sequence GYFADPKDPCKFYICSNWEAIHKSCPGNTRWNEKELTCT (SEQ ID No. 48).