Materials and methods for increasing the tocopherol content in seed oil
AssigneeBASF Plant Science GmbH
InventorCarl Andre
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Abstract
The present invention relates generally to the field of molecular biology and concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. The present invention also relates to methods for the manufacture of oil, fatty acid- or lipids-containing compositions, and to such oils and lipids as such.
Claims10
A method for increasing total tocopherol content of a plant relative to a control plant, comprising:
- (a) providing a transgenic plant transformed with multiple expression cassettes comprising transgenes which comprise at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase;
- and
- (b) obtaining transgenic seed oil from the transgenic plant, wherein said transgenic seed oil comprises increased n-3 very long chain polyunsaturated fatty acid (VLC-PUFA) content as compared to a control plant of the same species, wherein the increased n-3 VLC-PUFA content comprises EPA, DPA, and/or DHA;
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- (c) measuring total tocopherol content in said transgenic seed oil, wherein said transgenic seed oil has increased total tocopherol content as compared to a control plant of the same species, wherein the transgenic plant is an oilseed plant of the genus Brassica, and wherein expression of said transgenes in said transgenic plant results in said increase in total tocopherol content in said transgenic seed oil.
Dependencies are inferred from the claim text.