Back to results
US 11,771,111 B2Granted
Purified protein
AssigneeImpossible Foods Inc.
InventorsCarl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
Life of this patent
Estimating the term…
Abstract
Described herein are methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
Claims20
A protein composition produced by a method comprising:
- a) washing an aqueous suspension of a plurality of cells to yield a washed aqueous suspension;
- b) lysing the washed aqueous suspension of the plurality of cells to obtain a cell lysate;
- and
- c) filtering the cell lysate to obtain the protein composition;
- wherein steps a), b), and c) independently, are performed at a pH between about 8.5 and about 12.0.
2 more elements
A protein composition produced by a method comprising:
- a) lysing an aqueous suspension of a plurality of cells to obtain a cell lysate;
- and
- b) filtering the cell lysate to obtain the protein composition;
- wherein steps a) and b) independently, are performed at a pH between about 8.5 and about 12.0, and wherein the protein composition has a buffering capacity of less than about 2.5 mmol NaOH per gram dry solids.
1 more element
A protein composition produced by a method comprising:
- a) lysing an aqueous suspension of a plurality of cells to obtain a cell lysate;
- and
- b) filtering the cell lysate to obtain the protein composition;
- wherein steps a) and b) independently, are performed at a pH between about 8.5 and about 12.0, and
- wherein H2S is detectable in an amount of less than about 0.1 ppm after about 24 hours at 25° C. when L-cysteine is not added to 5 mL of a 2% (w/v) suspension of the protein composition at pH 7.0 and/or wherein H2S is detectable in an amount of at least about 0.2 ppm about 24 hours at 25° C. after 5 mL of a 2% (w/v) suspension of the protein composition is brought to about 25 mM final concentration of L-cysteine.
2 more elements
Dependencies are inferred from the claim text.