Systems and methods for an integrated holographic reconstruction tool with optical distortion removal and de-twinning

A system for reconstructing an image of an object from a hologram of the object, the system comprising:

• a user interface that receives a first uploading of a testing hologram of a testing pattern, a second uploading of a target hologram of an object and a selection of a reference beam hologram, and one or more reconstruction parameters including a normalization type configured by a user;
• a memory that stores a plurality of processor-executable instructions for image reconstruction from holograms;
• and
• one or more processors that execute the plurality of processor-executable instructions to perform operations comprising:
• reconstructing a testing image from the testing hologram according to the one or more reconstruction parameters, wherein the normalization type includes a subtraction division plus mean (SDPM) procedure including subtracting the reference beam hologram from the target hologram and dividing a result from the subtraction by the reference beam hologram plus a mean value of the reference beam hologram;
• iteratively re-generating the testing image while adjusting a propagation depth parameter until the testing pattern on the testing image comes within a focus threshold;
• and
• reconstructing an undistorted image of the object from the target hologram and the reference beam hologram according to the one or more reconstruction parameters including the adjusted propagation depth parameter.

A non-transitory processor-readable storage medium storing a plurality of processor-executable instructions for reconstructing an image of an object from a hologram of the object, the instructions being executable by a processor to perform operations comprising:

• receiving, via a user interface, one or more reconstruction parameters configured by a user;
• calibrating the one or more reconstruction parameters with a testing hologram until a resulting testing image comes within a focus threshold;
• obtaining, via a user interface, a target hologram of an object and a selection of a reference beam hologram;
• and
• reconstructing the image of the object from the target hologram and the reference beam hologram according to the one or more calibrated reconstruction parameters and a de-twinning setting that suppresses a virtual image in reconstruction wherein the one or more reconstruction parameters includes a subtraction division plus mean (SDPM) procedure conducted by:
• subtracting the reference beam hologram from the target hologram;
• and
• dividing a result from the subtraction by the reference beam hologram plus a mean value of the reference beam hologram.